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Impact of goat meat extract on physical barrier function and skin hydration. HaCaT cells were exposed to goat meat extract at concentrations of 50, 100, and 200 µg/mL for 24 h. Levels of ( a ) filaggrin, ( b ) aquaporin, and ( c ) hyaluronic acid were assessed using ELISA. The results are presented as mean ± SD from three independent experiments. * p < 0.05, ** p < 0.01.

Journal: Foods

Article Title: Skin Function Improvement and Anti-Inflammatory Effects of Goat Meat Extract

doi: 10.3390/foods13233934

Figure Lengend Snippet: Impact of goat meat extract on physical barrier function and skin hydration. HaCaT cells were exposed to goat meat extract at concentrations of 50, 100, and 200 µg/mL for 24 h. Levels of ( a ) filaggrin, ( b ) aquaporin, and ( c ) hyaluronic acid were assessed using ELISA. The results are presented as mean ± SD from three independent experiments. * p < 0.05, ** p < 0.01.

Article Snippet: These supernatants were then analyzed for TNFα (Elabscience, Houston, TX, USA), IL-1 (Elabscience), IL-6 (Elabscience), CTSS (MYbiosource), MDC/CCL22 (MYbiosource), RANTES/CCL5 (MYbiosource), TARC/CCL17 (MYbiosource), filaggrin (MYbiosource), aquaporin (MYbiosource), and hyaluronic acid (Elabscience) using ELISA kits, following the manufacturer’s instructions.

Techniques: Enzyme-linked Immunosorbent Assay

Urine HA, CD44 and RHAMM levels. Urine HA, CD44 and RHAMM levels were measured by ELISA from 99 diabetic patients with or without DKD. Comparisons among NRG (eGFR ≥90 mL/min/1.73 m 2 ), MRG (60 ≤ eGFR <90 mL/min/1.73 m 2 ) and SRG (eGFR <60 mL/min/1.73 m 2 ) groups were analyzed using one-way ANOVA followed by Tukey's multiple comparison test. Urine HA ( A ) and CD44 ( B ) as well as RHAMM ( C ) were significantly higher in MRG than in NRG and significantly higher in SRG than in NRG and MRG. ∗∗∗∗ P < .0001.

Journal: Clinical Kidney Journal

Article Title: Elevated RHAMM as a biomarker for predicting diabetic kidney disease in patients with type 2 diabetes

doi: 10.1093/ckj/sfae196

Figure Lengend Snippet: Urine HA, CD44 and RHAMM levels. Urine HA, CD44 and RHAMM levels were measured by ELISA from 99 diabetic patients with or without DKD. Comparisons among NRG (eGFR ≥90 mL/min/1.73 m 2 ), MRG (60 ≤ eGFR <90 mL/min/1.73 m 2 ) and SRG (eGFR <60 mL/min/1.73 m 2 ) groups were analyzed using one-way ANOVA followed by Tukey's multiple comparison test. Urine HA ( A ) and CD44 ( B ) as well as RHAMM ( C ) were significantly higher in MRG than in NRG and significantly higher in SRG than in NRG and MRG. ∗∗∗∗ P < .0001.

Article Snippet: Concentrations of plasma and urine HA, CD44 and RHAMM were measured with enzyme-linked immunosorbent assay (ELISA) kit, according to protocols provided by the manufacturer [Human Cluster of Differentiation (CD44) ELISA Kit; lot no. CSB-E11846h; CUSABIO, Wuhan, China; Human Hyaluronic acid (HA) ELISA Kit; lot no. CSB-E04805h; CUSABIO, Wuhan, China; and Human Hyaluronan Mediated Motility Receptor (HMMR) ELISA Kit, lot no. CSB-E04805h; Cloud-Clone Corp, Wuhan, China].

Techniques: Enzyme-linked Immunosorbent Assay, Comparison

Plasma HA, CD44 and RHAMM levels. Plasma HA, CD44 and RHAMM levels were measured by ELISA from 99 diabetic patients with or without DKD. Comparisons among NRG (eGFR ≥90 mL/min/1.73 m 2 ), MRG (60 ≤ eGFR <90 mL/min/1.73 m 2 ) and SRG (eGFR <60 mL/min/1.73 m 2 ) groups were analyzed using one-way ANOVA followed by Tukey's multiple comparison test. Plasma HA ( A ) was significantly higher in SRG than in NRG and MRG; plasma CD44 ( B ) was significantly higher in SRG than in NRG and MRG; plasma RHAMM ( C ) was significantly higher in MRG than in NRG and significantly higher in SRG than in NRG and MRG. ∗∗ P < .01 and ∗∗∗∗ P < .0001.

Journal: Clinical Kidney Journal

Article Title: Elevated RHAMM as a biomarker for predicting diabetic kidney disease in patients with type 2 diabetes

doi: 10.1093/ckj/sfae196

Figure Lengend Snippet: Plasma HA, CD44 and RHAMM levels. Plasma HA, CD44 and RHAMM levels were measured by ELISA from 99 diabetic patients with or without DKD. Comparisons among NRG (eGFR ≥90 mL/min/1.73 m 2 ), MRG (60 ≤ eGFR <90 mL/min/1.73 m 2 ) and SRG (eGFR <60 mL/min/1.73 m 2 ) groups were analyzed using one-way ANOVA followed by Tukey's multiple comparison test. Plasma HA ( A ) was significantly higher in SRG than in NRG and MRG; plasma CD44 ( B ) was significantly higher in SRG than in NRG and MRG; plasma RHAMM ( C ) was significantly higher in MRG than in NRG and significantly higher in SRG than in NRG and MRG. ∗∗ P < .01 and ∗∗∗∗ P < .0001.

Article Snippet: Concentrations of plasma and urine HA, CD44 and RHAMM were measured with enzyme-linked immunosorbent assay (ELISA) kit, according to protocols provided by the manufacturer [Human Cluster of Differentiation (CD44) ELISA Kit; lot no. CSB-E11846h; CUSABIO, Wuhan, China; Human Hyaluronic acid (HA) ELISA Kit; lot no. CSB-E04805h; CUSABIO, Wuhan, China; and Human Hyaluronan Mediated Motility Receptor (HMMR) ELISA Kit, lot no. CSB-E04805h; Cloud-Clone Corp, Wuhan, China].

Techniques: Clinical Proteomics, Enzyme-linked Immunosorbent Assay, Comparison